Difference between revisions of "Part:BBa I723020:Design"
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− | <partinfo> | + | <partinfo>BBa_I723020 SequenceAndFeatures</partinfo> |
===Design Notes=== | ===Design Notes=== | ||
− | + | This promoter can go in front of any gene you want expressed in response to the presence of BTEX compounds, provided you also have the XylR+Pr component | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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+ | Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by ''Pseudomonas putida'' (''arvilla'') MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13. | ||
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+ | Harayama, S., Leppik, R.A., Rekik, M., Mermod, N., Lehrbach, P.R., Reineke, W., and Timmis, K.N. 1986. Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product. Journal of Bacteriology 167, 455-461. |
Latest revision as of 17:17, 25 October 2007
Pu
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 195
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter can go in front of any gene you want expressed in response to the presence of BTEX compounds, provided you also have the XylR+Pr component
Source
Cloned from the TOL plasmid from Pseudomonas putida MT2. This is the promoter that regulates the TOL catabolic plasmid upper pathway operon.
References
Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.
Harayama, S., Leppik, R.A., Rekik, M., Mermod, N., Lehrbach, P.R., Reineke, W., and Timmis, K.N. 1986. Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product. Journal of Bacteriology 167, 455-461.