Difference between revisions of "Part:BBa I723021:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | This system contains a PstI site and needs site directed mutagenesis. It requires the presence of the responsive promoter Pu primary to allow exprssion of a desired gene after exposure to BTEX compounds | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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+ | Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by ''Pseudomonas putida'' (''arvilla'') MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13. |
Latest revision as of 17:13, 25 October 2007
XylR transcriptional regulator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1087
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 910
Illegal PstI site found at 1087 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 530
Illegal BglII site found at 1992 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1087
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1087
Illegal NgoMIV site found at 329
Illegal NgoMIV site found at 696 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1183
Design Notes
This system contains a PstI site and needs site directed mutagenesis. It requires the presence of the responsive promoter Pu primary to allow exprssion of a desired gene after exposure to BTEX compounds
Source
Cloned from the TOL plasmid from Pseudomonas putida MT2. The terminators were added separately.
References
Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.