Difference between revisions of "Part:BBa K2448022:Design"

Latest revision as of 21:13, 25 November 2017

D-Psicose 3-epimerase (Dpe) from Agrobacterium tumefaciens

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 190
Illegal NgoMIV site found at 250
Illegal NgoMIV site found at 601
Illegal AgeI site found at 628
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 156

Design Notes

Source

PCR amplification of the Atu4750 gene from the Agrobacterium tumefaciens str. C58 genome.

References

[1] Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK. Characterization of an Agrobacterium tumefaciens D-psicose 3-epimerase that converts D-fructose to D-psicose. Appl Environ Microbiol. (2006) 72, 981-985.

[2] Kim K, Kim HJ, Oh DK, Cha SS, Rhee S. Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes. J Mol Biol. (2006) 361, 920-931.

Revision as of 15:06, 29 October 2017 (view source) Brumake (Talk \| contribs) ← Older edit		Latest revision as of 21:13, 25 November 2017 (view source) IPopescu (Talk \| contribs)
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	[1] Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK. Characterization of an ''Agrobacterium tumefaciens'' D-psicose 3-epimerase that converts D-fructose to D-psicose. Appl Environ Microbiol. (2006) 72, 981-985.		[1] Kim HJ, Hyun EK, Kim YS, Lee YJ, Oh DK. Characterization of an ''Agrobacterium tumefaciens'' D-psicose 3-epimerase that converts D-fructose to D-psicose. Appl Environ Microbiol. (2006) 72, 981-985.

−	[2] Kim K, Kim HJ, Oh DK, Cha SS, Rhee S. Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes. J Mol Biol. (2006) 361, 920-931.	+	[2] Kim K, Kim HJ, Oh DK, Cha SS, Rhee S. Crystal structure of D-psicose 3-epimerase from ''Agrobacterium tumefaciens'' and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes. J Mol Biol. (2006) 361, 920-931.