Difference between revisions of "Part:BBa I723132:Design"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_I723132 short</partinfo>
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<partinfo>BBa_I723017 short</partinfo>
  
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<partinfo>BBa_I723132 SequenceAndFeatures</partinfo>
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<partinfo>BBa_I723017 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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[Christine/Maia]
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Site directed mutagenesis required to remove PstI site for full use of suffix and prefix when assembling this part next to others. this Part does not exist on its own, but as a composite with its native promoter Pr
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−
 
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===Source===
 
===Source===
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Cloned from the TOL plasmid from ''Pseudomonas putida'' MT2
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Cloned from the TOL plasmid from ''Pseudomonas putida'' MT2.
  
 
===References===
 
===References===
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Worsey, M., and Williams, P., 1975. Metabolism of toluene and xylenes by ''Pseudomonas putida'' (''arvilla'') MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.
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Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by ''Pseudomonas putida'' (''arvilla'') MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.

Latest revision as of 17:07, 25 October 2007


XylR coding region


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 643
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 466
    Illegal PstI site found at 643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 86
    Illegal BglII site found at 1548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 643
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 643
    Illegal NgoMIV site found at 252
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 739


Design Notes

Site directed mutagenesis required to remove PstI site for full use of suffix and prefix when assembling this part next to others. this Part does not exist on its own, but as a composite with its native promoter Pr

Source

Cloned from the TOL plasmid from Pseudomonas putida MT2.

References

Worsey, M., and Williams, P. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) MT-2: evidence for a new function of the TOL plasmid. J Bacteriol 124, 7-13.