Difference between revisions of "Part:BBa K2375001"
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<partinfo>BBa_K2375001 short</partinfo> | <partinfo>BBa_K2375001 short</partinfo> | ||
− | mCherry was optimized for E. coli usage. Also, the internal RBS that had been causing problems was removed. To do this, residue 7 was mutated from Glu (GAG) to Ser (TCT). The part sequence was based on BBa_J06504 | + | mCherry was optimized for E. coli usage. Also, the internal RBS that had been causing problems was removed. To do this, residue 7 was mutated from Glu (GAG) to Ser (TCT). The part sequence was based on [[Part:BBa_J06504|BBa_J06504]]. |
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Latest revision as of 20:27, 30 October 2017
mCherry Optimized, no internal RBS
mCherry was optimized for E. coli usage. Also, the internal RBS that had been causing problems was removed. To do this, residue 7 was mutated from Glu (GAG) to Ser (TCT). The part sequence was based on BBa_J06504.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]