Difference between revisions of "Part:BBa K2207010"
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Promoter(phlF regulated) | Promoter(phlF regulated) | ||
| − | This part is an inducible promoter regulated by PhlF. | + | This part is an inducible promoter regulated by PhlF. And the sequence of it is offered by Jef D.Boeke's laboratory. |
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It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens | It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens | ||
and related species. | and related species. | ||
Latest revision as of 14:49, 30 October 2017
phlo Promoter
BBa_K2207010
Promoter(phlF regulated)
This part is an inducible promoter regulated by PhlF. And the sequence of it is offered by Jef D.Boeke's laboratory.
It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens and related species.
In the absence of PhlF protein, this part promotes transcription.
In the presence of PhlF protein, this part inhibits transcription.
PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).
PhlF can be coded by BBa_K2207009.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 807
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]