Difference between revisions of "Part:BBa K2207010"
 
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BBa_K2207010
 
BBa_K2207010
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Promoter(phlF regulated)
 
Promoter(phlF regulated)
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This part is an inducible promoter regulated by PhlF.
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It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens and related species.
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This part is an inducible promoter regulated by PhlF. And the sequence of it is offered by Jef D.Boeke's laboratory.
 +
 
 +
It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens
 +
and related species.
  
 
In the absence of PhlF protein, this part promotes transcription.
 
In the absence of PhlF protein, this part promotes transcription.
 +
 
In the presence of PhlF protein, this part inhibits transcription.
 
In the presence of PhlF protein, this part inhibits transcription.
 +
 
PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).
 
PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).
 +
 
PhlF can be coded by BBa_K2207009.
 
PhlF can be coded by BBa_K2207009.
  
  
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Usage and Biology
 
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The sequence of this part is designed and offered by Jef D. Boeke’s laboratory. And we used the mailed plasmid to test its function. As we can see from the figure below, it is obvious that this modified promoter can  transcribe the CDS normally without the phlF in the Saccharomyces cerevisiaea.
 
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https://static.igem.org/mediawiki/2017/2/27/ZJU_China_CT_what.jpg
 
  
  

Latest revision as of 14:49, 30 October 2017


phlo Promoter

BBa_K2207010

Promoter(phlF regulated)

This part is an inducible promoter regulated by PhlF. And the sequence of it is offered by Jef D.Boeke's laboratory.

It is a modified ADH1 promoter from the yeast Saccharomyces cerevisiaea having a phlF operator sequence (phlO) embedded downstream the promoter. The phlO region can be bound by phlF, a regulatory protein existing in Pseudomonas fluorescens and related species.

In the absence of PhlF protein, this part promotes transcription.

In the presence of PhlF protein, this part inhibits transcription.

PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).

PhlF can be coded by BBa_K2207009.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 807
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]