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===<span class='h3bb'>Sequence and Features</span>===
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<partinfo>BBa_K2382001 SequenceAndFeatures</partinfo>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2382012 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2382001 parameters</partinfo>
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<partinfo>BBa_K2382012 parameters</partinfo>
 
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===Usage and Biology===
 
===Usage and Biology===
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A rigid linker link two proteins to form one fusion protein. It can prevent the
 
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two proteins from interrupting each other while folding.
 
  
  
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The (EAAAK)5 linker is shown to possess a pH-dependent auto-cleavage feature. In the range pH 6–7, the
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This part is a rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK
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target protein becomes automatically released from the fusion protein without proteolytic treatment.
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three times to maintain distance of two proteins and preventing from interrupting each other
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Although the mechanism of this auto-cleavage property of an (EAAAK)5 linker is unclear, this feature has
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while folding.<br>
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been successfully employed for many cases of protein purification without the tag of a fusion protein.
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The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently. <br>
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In our fusion protein design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the
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two proteins from interrupting each other while folding. And the computer structure simulation support this description.
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__TOC__
  
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===Characterization of the EAAAK===
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==Characterization of the EAAAK==
  
 
===References===
 
===References===

Latest revision as of 22:09, 1 November 2017

EAAAK


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This part is a rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK three times to maintain distance of two proteins and preventing from interrupting each other while folding.
The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently.

In our fusion protein design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding. And the computer structure simulation support this description.


Characterization of the EAAAK

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021