Difference between revisions of "Part:BBa I723024"
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+ | This is the coding region for the first of two ''Pseudomonas aeruginosa'' PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin. | ||
+ | |||
+ | ===Information contributed by City of London UK (2021)=== | ||
+ | Part information is collated here to help future users of the BioBrick registry. | ||
+ | |||
+ | Metadata: | ||
+ | *'''Group:''' City of London UK 2021 | ||
+ | *'''Author:''' Janusan Jeyananthan | ||
+ | *'''Summary:''' Added information collated from existing scientific studies | ||
+ | |||
+ | ---- | ||
+ | |||
+ | PhzS required for activity in vitro. | ||
+ | |||
+ | Enzyme catalytic activity: phenazine-1-carboxylate + S-adenosyl-L-methionine = 5-methyl-phenazine-1-carboxylate + S-adenosyl-L-homocysteine | ||
+ | <ref>"phzM" n.d Uniprot. Accessed October 3, 2021. https://www.uniprot.org/uniprot/Q9HWH2</ref> | ||
+ | [[File:phzM Reaction Diagram.JPG|x200px|center]] | ||
+ | |||
+ | ===References=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Latest revision as of 12:55, 3 October 2021
PhzM
This is the coding region for the first of two Pseudomonas aeruginosa PA01 enzymes required to convert phenazine-1-carboxylic acid (PCA) into pyocyanin.
Information contributed by City of London UK (2021)
Part information is collated here to help future users of the BioBrick registry.
Metadata:
- Group: City of London UK 2021
- Author: Janusan Jeyananthan
- Summary: Added information collated from existing scientific studies
PhzS required for activity in vitro.
Enzyme catalytic activity: phenazine-1-carboxylate + S-adenosyl-L-methionine = 5-methyl-phenazine-1-carboxylate + S-adenosyl-L-homocysteine [1]
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 206
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 424
Illegal NgoMIV site found at 428
Illegal NgoMIV site found at 787 - 1000COMPATIBLE WITH RFC[1000]
- ↑ "phzM" n.d Uniprot. Accessed October 3, 2021. https://www.uniprot.org/uniprot/Q9HWH2