Difference between revisions of "Part:BBa K1033121"

Latest revision as of 09:51, 29 October 2017

Miraculin

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.

Part improvement: team Tel-Hai 2017

Group: Tel-Hai 2017

Author: Almog Angel

BBa_K2408023

Miraculin is a glycoprotein made by plant cells, therefore a yeast like S. cerevisiae would make a better expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features:
1. ADH1 - strong constitutive promoter for S. cerevisiae.
2. His tag for purification.
3. ADH1 terminator for more effective expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 20: / Line 20: @@
 Miraculin is a glycoprotein made by plant cells, therefore a yeast like <I>S. cerevisiae</I> would make a better
-expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: 1. ADH1 - strong constitutive promoter for  <I>S. cerevisiae</I>. 2. His tag for purification 3. ADH1 terminator for more effective expression.
+expression host than bacteria. We codon optimize the part for yeast expression. In Addtion, we added few more features: <br>1. ADH1 - strong constitutive promoter for  <I>S. cerevisiae</I>.<br> 2. His tag for purification. <br>3. ADH1 terminator for more effective expression.
 <!-- Add more about the biology of this part here