Difference between revisions of "Part:BBa K2325101"
 
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Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.
 
Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2325101 SequenceAndFeatures</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
 
Using the pGEX-2T vector, the FOR could be expressed in BL21(DE3).
 
Using the pGEX-2T vector, the FOR could be expressed in BL21(DE3).
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[[File:BBa_K2325101--1.png|thumb|left|400px|""Figure 1."" Growth curves of <i>E.coli</i> containing formamidase gene in a basal MOPS medium in the presence of formamide as the nitrogen source comparing with negative controls]]<br />
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[[File:BBa_K2325101--1.png|750px]]<br />
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<b>Figure 1. Growth curves of <i>E.coli</i> containing formamidase gene in a basal MOPS medium in the presence of formamide as the nitrogen source comparing with negative controls</b>
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To verify that our recombinant <i>E. coli</i> containing formamidase gene could work efficiently to utilize formamide as its nitrogen sources, we cultivated the recombinant one and the negative controls in a basal MOPS medium with formamide as the sole nitrogen sources.
 
To verify that our recombinant <i>E. coli</i> containing formamidase gene could work efficiently to utilize formamide as its nitrogen sources, we cultivated the recombinant one and the negative controls in a basal MOPS medium with formamide as the sole nitrogen sources.
 
<i>E. coli</i> containing formamidase gene, reached maximum concentration after 48 hours in the basal MOPS medium in the presence of 200 mM formamide. While <i>E.coli</i> without formamidase gene basically did not grow.
 
<i>E. coli</i> containing formamidase gene, reached maximum concentration after 48 hours in the basal MOPS medium in the presence of 200 mM formamide. While <i>E.coli</i> without formamidase gene basically did not grow.
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K2325101 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 13:32, 29 October 2017


Formamidase expression device

The BBa_K2325101 contains formamidase gene with T7 promoter and terminator.

Formamidase is an enzyme involved in the production of ammonia for providing nitrogen source for the engineering bacteria. This part has been constructed into expression vectors to modify the metabolic pathway of nitrogen in E.coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 189
    Illegal NgoMIV site found at 676
    Illegal NgoMIV site found at 871
    Illegal NgoMIV site found at 1018
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Using the pGEX-2T vector, the FOR could be expressed in BL21(DE3).

""Figure 1."" Growth curves of E.coli containing formamidase gene in a basal MOPS medium in the presence of formamide as the nitrogen source comparing with negative controls

To verify that our recombinant E. coli containing formamidase gene could work efficiently to utilize formamide as its nitrogen sources, we cultivated the recombinant one and the negative controls in a basal MOPS medium with formamide as the sole nitrogen sources. E. coli containing formamidase gene, reached maximum concentration after 48 hours in the basal MOPS medium in the presence of 200 mM formamide. While E.coli without formamidase gene basically did not grow.