Difference between revisions of "Part:BBa K2486004"
 
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# A synthetic terminator ([https://parts.igem.org/Part:BBa_K2486006 BBa_K2486006]).
 
# A synthetic terminator ([https://parts.igem.org/Part:BBa_K2486006 BBa_K2486006]).
  
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We built this part in order to constitutively inhibit our synthetic promoter, the P<sub>dtxR</sub> wich is best described in parts ([https://parts.igem.org/Part:BBa_K2486023 BBa_K2486023]), ([https://parts.igem.org/Part:BBa_K2486014 BBa_K2486014)] and ([https://parts.igem.org/Part:BBa_K2486009 BBa_K2486009)].
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We built this part in order to constitutively produce the dtxR regulator for our synthetic promoter, the P<sub>dtxR</sub> wich is best described in parts ([https://parts.igem.org/Part:BBa_K2486023 BBa_K2486023]), ([https://parts.igem.org/Part:BBa_K2486014 BBa_K2486014)] and ([https://parts.igem.org/Part:BBa_K2486009 BBa_K2486009)].
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 14:56, 29 June 2019


dtxR generator

A part that constitutively generates the DtxR repressor.

Usage and Biology

The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

  1. A promoter (BBa_J23108) from Anderson promoter collection.
  2. A ribosome binding site (BBa_B0034).
  3. A short spacer of 6bp (BBa_K2486024).
  4. dtxR gene (BBa_K2486005).
  5. A short spacer of 16bp (BBa_K2486024).
  6. A synthetic terminator (BBa_K2486006).

We built this part in order to constitutively produce the dtxR regulator for our synthetic promoter, the PdtxR wich is best described in parts (BBa_K2486023), (BBa_K2486014) and (BBa_K2486009).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]