Difference between revisions of "Part:BBa K2448001:Design"
 
(One intermediate revision by the same user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This sequence has been specifically optimised for ''Escherichia coli'' K12 with help of [http://www.jcat.de/ JCAT]. Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.
+
This sequence has been specifically optimised for ''Escherichia coli'' K12 with the help of [http://www.jcat.de/ JCAT]. Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.
  
 
===Source===
 
===Source===
Line 16: Line 16:
  
 
[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the ''Aequorea victoria'' green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.
 
[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the ''Aequorea victoria'' green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.
−
 
−
[2] Addgene plasmid # 53976
 

Latest revision as of 18:53, 31 December 2018


mEmerald


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been specifically optimised for Escherichia coli K12 with the help of [http://www.jcat.de/ JCAT]. Before optimization, the CAI-Value of the original sequence was 0.419 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 48% GC-Content.

Source

DNA synthesis

References

[1] Cubitt AB, Woollenweber LA, Heim R. Understanding structure-function relationships in the Aequorea victoria green fluorescent protein. Methods Cell Biol. (1999) 58, 19–30.