Difference between revisions of "Part:BBa K2486004"

Latest revision as of 14:56, 29 June 2019

dtxR generator

A part that constitutively generates the DtxR repressor.

Usage and Biology

The BBa_K486004 is a dtxR constitutive generator. This part is composed of:

A promoter (BBa_J23108) from Anderson promoter collection.
A ribosome binding site (BBa_B0034).
A short spacer of 6bp (BBa_K2486024).
dtxR gene (BBa_K2486005).
A short spacer of 16bp (BBa_K2486024).
A synthetic terminator (BBa_K2486006).

We built this part in order to constitutively produce the dtxR regulator for our synthetic promoter, the P_dtxR wich is best described in parts (BBa_K2486023), (BBa_K2486014) and (BBa_K2486009).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 509
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-BBa_J23108 BBa_B0034 BBa_K2486024 BBa_K2486005 BBa_K2486025 BBa_K2486006
 The BBa_K486004 is a dtxR constitutive generator. This part is composed of:
@@ Line 17: / Line 16: @@
 # A synthetic terminator ([https://parts.igem.org/Part:BBa_K2486006 BBa_K2486006]).
-We built this part in order to constitutively inhibit our synthetic promoter, the P<sub>dtxR</sub> wich is best described in parts BBa_K2486023, BBa_K2486014 and BBa_K2486009.
+We built this part in order to constitutively produce the dtxR regulator for our synthetic promoter, the P<sub>dtxR</sub> wich is best described in parts ([https://parts.igem.org/Part:BBa_K2486023 BBa_K2486023]), ([https://parts.igem.org/Part:BBa_K2486014 BBa_K2486014)] and ([https://parts.igem.org/Part:BBa_K2486009 BBa_K2486009)].
 <span class='h3bb'>Sequence and Features</span>