Difference between revisions of "Part:BBa K2448009:Design"
 
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===Design Notes===
 
===Design Notes===
  
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This sequence has been further optimised for high expression in ''Escherichia coli'' K12 with help of JCAT (http://www.jcat.de/). The BsmBI site was also removed.
+
This sequence has been further optimised for high expression in ''Escherichia coli'' K12 with help of [http://www.jcat.de/ JCAT]. The BsmBI site was also removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]]).
  
 
===Source===
 
===Source===
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[1] Ramseier TM, Bledig S, Michotey V, Feghali R, Saier MH Jr. The global regulatory protein FruR modulates the direction of carbon flow in ''Escherichia coli''. Mol Microbiol. (1995) 16, 1157-1169.
 
[1] Ramseier TM, Bledig S, Michotey V, Feghali R, Saier MH Jr. The global regulatory protein FruR modulates the direction of carbon flow in ''Escherichia coli''. Mol Microbiol. (1995) 16, 1157-1169.
 +
 +
[2] Shimada T, Yamamoto K, Ishihama A. Novel members of the Cra regulon involved in carbon metabolism in ''Escherichia coli''. J Bacteriol. (2011) 193, 649-659.

Latest revision as of 22:09, 28 October 2017


FruR from Escherichia coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been further optimised for high expression in Escherichia coli K12 with help of [http://www.jcat.de/ JCAT]. The BsmBI site was also removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024).

Source

DNA synthesis

References

[1] Ramseier TM, Bledig S, Michotey V, Feghali R, Saier MH Jr. The global regulatory protein FruR modulates the direction of carbon flow in Escherichia coli. Mol Microbiol. (1995) 16, 1157-1169.

[2] Shimada T, Yamamoto K, Ishihama A. Novel members of the Cra regulon involved in carbon metabolism in Escherichia coli. J Bacteriol. (2011) 193, 649-659.