Difference between revisions of "Part:BBa K2448009"

Latest revision as of 19:07, 28 October 2017

FruR from Escherichia coli

This part is the FruR (Cra) found in Escherichia coli K-12 (ECK0081).

Usage and Biology

FruR is transcriptional repressor-activator for carbon metabolism [1, 2]. It activates transcription of genes involved in Krebs cycle, glyoxylate shunt and gluconeogenesis and represses genes involved in sugar catabolism, such as FruB.

Our team used this transcription factor together with the pFruB promoter (BBa_K2448017) to build a Fructose biosensor (BBa_K2448032). The results show that FruR behaved as predicted under and without induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2448009 short</partinfo>
-This part is the FruR found in Escherichia coli K-12 (ECK0081). The sequence was modified
+This part is the FruR (Cra) found in ''Escherichia coli'' K-12 (ECK0081).
 ===Usage and Biology===
-FruR is transcriptional repressor-activator for carbon metabolism. It activates transcription of genes involved in Krebs cycle, glyoxylate shunt and gluconeogenesis and represses genes involved in sugar catabolism, such as FruB.
+FruR is transcriptional repressor-activator for carbon metabolism [1, 2]. It activates transcription of genes involved in Krebs cycle, glyoxylate shunt and gluconeogenesis and represses genes involved in sugar catabolism, such as FruB.
-Our team used this transcription factor together with the FruB promoter (pFruB, [[Part:BBa_K2448017|BBa_K2448017]]) to build a Fructose biosensor ([[Part:BBa_K2448032|BBa_K2448032]]) and the results shows that FruR behaved as predicted under and without induction.
+Our team used this transcription factor together with the pFruB promoter ([[Part:BBa_K2448017|BBa_K2448017]]) to build a Fructose biosensor ([[Part:BBa_K2448032|BBa_K2448032]]). The results show that FruR behaved as predicted under and without induction.
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