Difference between revisions of "Part:BBa K2448004:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | This codon optimized mCherry for Escherichia coli K12 is derived from ([[Part:BBa_J06504|BBa_J06504]]). The optimization has been performed with help of | + | This codon optimized mCherry for ''Escherichia coli'' K12 is derived from ([[Part:BBa_J06504|BBa_J06504]]). The optimization has been performed with help of [http://www.jcat.de/ JCAT]. |
Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]]) | Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis ([[Part:BBa_K2448023|BBa_K2448023]] and [[Part:BBa_K2448024|BBa_K2448024]]) |
Latest revision as of 21:54, 28 October 2017
mCherry
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 570
Illegal AgeI site found at 682 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This codon optimized mCherry for Escherichia coli K12 is derived from (BBa_J06504). The optimization has been performed with help of [http://www.jcat.de/ JCAT].
Before optimization, the CAI-Value of the original sequence was 0.387 with a 62% GC-Content. The optimization allows a CAI-Value for the improved sequence of 1.0 with a 47% GC-Content. NcoI and BbsI restrictions sites have also been removed to allow potential Golden Gate Assembly use and complementarity with the Universal Biosensing Chassis (BBa_K2448023 and BBa_K2448024)
Source
DNA synthesis