Difference between revisions of "Part:BBa K2271104:Design"

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===Design Notes===
 
===Design Notes===
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The membrane domain PEX13 (amino acids 200-310) was used as a peroxisomal membrane anchor for fluorescence proteins. We therefore added a triple GGGGS linker to allow an undisturbed c-terminal fusion.
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The membrane domain of Pex13 was desinged as a peroxisomal membrane anchor for proteins as previously described by <a href="https://www.ncbi.nlm.nih.gov/pubmed/15133130"><abbr title="Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals"> Erdmann et al. (2004)</abbr></a>. We only used the amino acids 200-310 and added a triple GGGGS linker to allow an undisturbed c-terminal fusion of any protein of interest.
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===Source===
 
===Source===
  
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===References===
 
===References===
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Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals - Erdmann <i>et al.</i>, 2004
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Latest revision as of 20:16, 1 November 2017


PEX13 membrane anchor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The membrane domain of Pex13 was desinged as a peroxisomal membrane anchor for proteins as previously described by Erdmann et al. (2004). We only used the amino acids 200-310 and added a triple GGGGS linker to allow an undisturbed c-terminal fusion of any protein of interest.

Source

UniProtKB - P80667 (PEX13_YEAST)

References

Peroxisomal Membrane Proteins Contain Common Pex19p-binding Sites that Are an Integral Part of Their Targeting Signals - Erdmann et al., 2004