Difference between revisions of "Part:BBa K2448016:Design"
(One intermediate revision by the same user not shown) | |||
Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
− | pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the binding sequence of a transcription factor, PsiR (BBa_K2448006). | + | pPsiTac1 is a synthetic promoter derived from pTacI ([[Part:BBa_K864400|BBa_K864400]]) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR ([[Part:BBa_K2448006|BBa_K2448006]]). |
===Source=== | ===Source=== | ||
Line 16: | Line 16: | ||
===References=== | ===References=== | ||
− | [1] de Boer | + | [1] de Boer HA, Comstock LJ, Vasser M. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci U S A (1983) 80, 21-25. |
− | [2] http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087 | + | [2] [http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087 RegPrecise database] |
Latest revision as of 22:16, 28 October 2017
pPsiTac1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR (BBa_K2448006).
Source
DNA synthesis
References
[1] de Boer HA, Comstock LJ, Vasser M. The tac promoter: A functional hybrid derived from the trp and lac promoters. Proc Natl Acad Sci U S A (1983) 80, 21-25. [2] [http://regprecise.lbl.gov/RegPrecise/sites.jsp?regulog_id=5087 RegPrecise database]