Difference between revisions of "Part:BBa K2448012"

Latest revision as of 21:41, 28 October 2017

pPsiA from Sinorhizobium fredii

This part is the promoter region (0.4 kb upstream) of the PsiA gene of Sinorhizobium fredii (NGR_b11530)

pPsiA is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiA promoter together with the Sinorhizobium fredii PsiR transcription factor (BBa_K2448007) to build a Psicose biosensor (BBa_K2448028). The results show that pPsiA is functional in E. coli and that it behaved as predicted under and without induction.

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Number of predicted promoters -      1
Promoter Pos:    235 LDF-  1.88
-10 box at pos.    219 cgatagtct Score    37
-35 box at pos.    197 ttgacg    Score    60
Oligonucleotides from known TF binding sites:
     argR2:  GCAAATAA at position     244 Score -   6

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 345
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2448012 short</partinfo>
-Promoter region (0.4 kb upstream) of PsiA gene of Sinorhizobium fredii (NGR_b11530)
+This part is the promoter region (0.4 kb upstream) of the PsiA gene of ''Sinorhizobium fredii'' (NGR_b11530)
+pPsiA is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) which is inhibited in the presence of D-Psicose [1].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Our team used this pPsiA promoter together with the ''Sinorhizobium fredii'' PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) to build a Psicose biosensor ([[Part:BBa_K2448028|BBa_K2448028]]). The results show that pPsiA is functional in ''E. coli'' and that it behaved as predicted under and without induction.
+ Using the BPROM webserver [2], the predicted features of this promoter are:
+ Threshold for promoters -  0.20
+ Number of predicted promoters -      1
+ Promoter Pos:    235 LDF-  1.88
+ -10 box at pos.    219 cgatagtct Score    37
+ -35 box at pos.    197 ttgacg    Score    60
+ Oligonucleotides from known TF binding sites:
+      argR2:  GCAAATAA at position     244 Score -   6
 <!-- -->