Difference between revisions of "Part:BBa K2448013"
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<partinfo>BBa_K2448013 short</partinfo> | <partinfo>BBa_K2448013 short</partinfo> | ||
− | + | This part is the promoter region (0.4 kb upstream) of the PsiR gene of ''Sinorhizobium fredii'' (NGR_b11520) | |
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+ | pPsiR is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) which is inhibited in the presence of D-Psicose [1]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | Our team used this pPsiR promoter together with the ''Sinorhizobium fredii'' PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) to build a Psicose biosensor ([[Part:BBa_K2448029|BBa_K2448029]]). The results show that pPsiR is functional in ''E. coli'' and that it behaved as predicted under and without induction. | ||
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+ | Using the BPROM webserver [2], the predicted features of this promoter are: | ||
+ | Threshold for promoters - 0.20 | ||
+ | Number of predicted promoters - 1 | ||
+ | Promoter Pos: 350 LDF- 1.71 | ||
+ | -10 box at pos. 335 gactatcct Score 43 | ||
+ | -35 box at pos. 314 ttgcac Score 33 | ||
+ | Oligonucleotides from known TF binding sites: | ||
+ | No such sites for promoter at 350 | ||
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Latest revision as of 21:41, 28 October 2017
pPsiR from Sinorhizobium fredii
This part is the promoter region (0.4 kb upstream) of the PsiR gene of Sinorhizobium fredii (NGR_b11520)
pPsiR is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].
Usage and Biology
Our team used this pPsiR promoter together with the Sinorhizobium fredii PsiR transcription factor (BBa_K2448007) to build a Psicose biosensor (BBa_K2448029). The results show that pPsiR is functional in E. coli and that it behaved as predicted under and without induction.
Using the BPROM webserver [2], the predicted features of this promoter are: Threshold for promoters - 0.20 Number of predicted promoters - 1 Promoter Pos: 350 LDF- 1.71 -10 box at pos. 335 gactatcct Score 43 -35 box at pos. 314 ttgcac Score 33 Oligonucleotides from known TF binding sites: No such sites for promoter at 350
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 211
- 1000COMPATIBLE WITH RFC[1000]