Difference between revisions of "Part:BBa K302035:Experience"

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===Applications of BBa_K302035===
 
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====iGEM2017 SZU-China confirmed the function of BBa_K302035.====
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We made characterization of this part in our chassis B.subtilis WB800.The sucrose was added in the culture medium when the value of OD<sub>600</sub> reach stationary phase initially, and the final concentration of sucrose was 20mmol/L. Then we recorded the variation of OD<sub>600</sub> by interval of 4 hours and the result is shown as follow.
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<center><html><img src='https://static.igem.org/mediawiki/parts/thumb/8/81/Characterization_of_MazEF-suicide_switch_in_Bacillus_subtilis_strain_WB800.png/800px-Characterization_of_MazEF-suicide_switch_in_Bacillus_subtilis_strain_WB800.png' ></html></center>
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Fig. The OD<sub>600</sub> of original strain WB800 and recombinant B.subtilis WB800_MazEF recorded by interval of 4 hours.The red arrow represents the adjunction of Sucrose (20mmol/L).
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For detail, see http://http://2017.igem.org/Team:SZU-China/Results

Latest revision as of 16:23, 1 November 2017


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Applications of BBa_K302035

User Review

iGEM2017 SZU-China confirmed the function of BBa_K302035.

We made characterization of this part in our chassis B.subtilis WB800.The sucrose was added in the culture medium when the value of OD600 reach stationary phase initially, and the final concentration of sucrose was 20mmol/L. Then we recorded the variation of OD600 by interval of 4 hours and the result is shown as follow.

Fig. The OD600 of original strain WB800 and recombinant B.subtilis WB800_MazEF recorded by interval of 4 hours.The red arrow represents the adjunction of Sucrose (20mmol/L). For detail, see http://http://2017.igem.org/Team:SZU-China/Results