Difference between revisions of "Part:BBa K2360007"
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It is special operon which can make a specific reponse to chromium ions. | It is special operon which can make a specific reponse to chromium ions. | ||
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<partinfo>BBa_K2360007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2360007 SequenceAndFeatures</partinfo> | ||
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We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions. | We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions. | ||
Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function. | Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function. | ||
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<br>Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions. | <br>Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions. | ||
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we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr<sup>6+</sup>, which showed this devices did not work as we expected. | we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr<sup>6+</sup>, which showed this devices did not work as we expected. | ||
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Latest revision as of 03:12, 28 October 2017
pChrB-ChrB
It is special operon which can make a specific reponse to chromium ions.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 136
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 911
- 1000COMPATIBLE WITH RFC[1000]
Figure 1. Bio-circuit of BBa_K2360007
Experiment
Lysis gene expression
We have developed the lysis module (BBa_K2360000) and five metal detecting module for E. coli BL21. Combining the lysis module and detecting module, we have constructed five devices which can respond to different metal ions.
Because the lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of our engineering bacteria in different concentration of metal ions to prove its function.
Figure 1. the growth curve of chromium detecting device in different concentration of chromium ions.
we measured the growth curve of our engineering bacteria in different concentration of chromium ions.It was a pity that we found the OD600 of E. coli with did not decline after we added Cr6+, which showed this devices did not work as we expected.