Difference between revisions of "Part:BBa K2360011"
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We can just transform the plasmid to E.coil BL21 to detect the lead ion. | We can just transform the plasmid to E.coil BL21 to detect the lead ion. | ||
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| − | = | + | <span class='h3bb'>Sequence and Features</span> |
| + | <partinfo>BBa_K2360011 SequenceAndFeatures</partinfo> | ||
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Figure 1. Bio-circuit of BBa_K2360011 | Figure 1. Bio-circuit of BBa_K2360011 | ||
| − | + | ===Experiment=== | |
| + | ==Lysis gene expression== | ||
Our device has an obvious contact to lead ions’ concentration.we measured the growth curve of our engineering bacteria in different concentration of lead ions to prove its function. | Our device has an obvious contact to lead ions’ concentration.we measured the growth curve of our engineering bacteria in different concentration of lead ions to prove its function. | ||
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Figure 2. the growth curve of lead detecting device in different concentration of mercury ions. | Figure 2. the growth curve of lead detecting device in different concentration of mercury ions. | ||
As shown in the growth curves of E. coli with lead detecting device, the growth rate of E. coli BL21 is decline obviously after adding the concentration of Pb<sup>2+</sup> from 10<sup>-7</sup>M to 10<sup>-5</sup>M, indicating that this device can detect the lead ions. | As shown in the growth curves of E. coli with lead detecting device, the growth rate of E. coli BL21 is decline obviously after adding the concentration of Pb<sup>2+</sup> from 10<sup>-7</sup>M to 10<sup>-5</sup>M, indicating that this device can detect the lead ions. | ||
| − | + | ==Chromogenic Reaction== | |
Our device has an obvious contact to lead ions’ concentration. By detecting the shading of the product’s color, we can qualitatively detect the concentration of lead metals. | Our device has an obvious contact to lead ions’ concentration. By detecting the shading of the product’s color, we can qualitatively detect the concentration of lead metals. | ||
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Figure 3. the comparison between ONPG and X-gal.c. lead detecting device’s chromogenic reaction at 20 mins. | Figure 3. the comparison between ONPG and X-gal.c. lead detecting device’s chromogenic reaction at 20 mins. | ||
| − | + | ==Specificity== | |
We verified the detecting devices specificity of lead ions.The result indicated that the lead detecting device has good specificity for specific metal ions. | We verified the detecting devices specificity of lead ions.The result indicated that the lead detecting device has good specificity for specific metal ions. | ||
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Figure 4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH<sub>2</sub>O | Figure 4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH<sub>2</sub>O | ||
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Latest revision as of 03:20, 28 October 2017
rrnB' terminater+PbrR+pPbrR+SRRz+rrnB terminater
It is a high-efficiency lead detecting device. This device consist two parts, the lysis module (BBa_K2360000) and lead detecting module(BBa_K2360006).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the lead ion.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Figure 1. Bio-circuit of BBa_K2360011
Experiment
Lysis gene expression
Our device has an obvious contact to lead ions’ concentration.we measured the growth curve of our engineering bacteria in different concentration of lead ions to prove its function.
Figure 2. the growth curve of lead detecting device in different concentration of mercury ions.
As shown in the growth curves of E. coli with lead detecting device, the growth rate of E. coli BL21 is decline obviously after adding the concentration of Pb2+ from 10-7M to 10-5M, indicating that this device can detect the lead ions.
Chromogenic Reaction
Our device has an obvious contact to lead ions’ concentration. By detecting the shading of the product’s color, we can qualitatively detect the concentration of lead metals.
Figure 3. the comparison between ONPG and X-gal.c. lead detecting device’s chromogenic reaction at 20 mins.
Specificity
We verified the detecting devices specificity of lead ions.The result indicated that the lead detecting device has good specificity for specific metal ions.
Figure 4. The growth of the E. coli with lead detecting device after adding different metal ions and ddH2O