Difference between revisions of "Part:BBa K2360012"
 
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This device consist two parts, the lysis module (BBa_K2360000) and chromium detecting module(BBa_K2360007).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function.
 
This device consist two parts, the lysis module (BBa_K2360000) and chromium detecting module(BBa_K2360007).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function.
 
We can just transform the plasmid to E.coil BL21 to detect the chromium ion.
 
We can just transform the plasmid to E.coil BL21 to detect the chromium ion.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2360012 SequenceAndFeatures</partinfo>
  
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<br>
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===Usage and Biology===
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[[File:SCUT_A gene.PNG]]
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<br>Figure 1. Bio-circuit of BBa_K2360012
 
<br>Figure 1. Bio-circuit of BBa_K2360012
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===Experiment===
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==Lysis gene expression==
 
We measured the growth curve of our engineering bacteria in different concentration of nickel ions. It was a pity that we found the OD600 of E. coli with did not decline after we added Cr<sup>6+</sup>, which showed this device did not work as we expected.
 
We measured the growth curve of our engineering bacteria in different concentration of nickel ions. It was a pity that we found the OD600 of E. coli with did not decline after we added Cr<sup>6+</sup>, which showed this device did not work as we expected.
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<br>
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[[File:SCUT_A PART CR.PNG]]
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<br>Figure 2. the growth curve of chromium detecting device in different concentration of chromium ions.
  
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<br>Figure 5. the growth curve of chromium detecting device in different concentration of chromium ions.
 
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K2360012 SequenceAndFeatures</partinfo>
 
  
  

Latest revision as of 03:21, 28 October 2017


pChrB-ChrB+SRRz+rrnB terminater

It is a high-efficiency chromium detecting device. This device consist two parts, the lysis module (BBa_K2360000) and chromium detecting module(BBa_K2360007).The lysis gene will be expressed when the detecting module makes a response to the metal ions, which will make bacterial cleavage, resulting in the decrease of OD600, we measured the growth curve of the engineering bacteria in different concentration of metal ions to prove its function. We can just transform the plasmid to E.coil BL21 to detect the chromium ion. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 911
  • 1000
    COMPATIBLE WITH RFC[1000]


SCUT A gene.PNG
Figure 1. Bio-circuit of BBa_K2360012

Experiment

Lysis gene expression

We measured the growth curve of our engineering bacteria in different concentration of nickel ions. It was a pity that we found the OD600 of E. coli with did not decline after we added Cr6+, which showed this device did not work as we expected.

SCUT A PART CR.PNG
Figure 2. the growth curve of chromium detecting device in different concentration of chromium ions.