Difference between revisions of "Part:BBa I751021"
 
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<partinfo>BBa_I751021 short</partinfo>
 
<partinfo>BBa_I751021 short</partinfo>
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This is made for A-side plasmid in our project.
 
  
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Regulated by lac repressor, this part makes LuxI, cI repressor, and mCherry (for detecting).
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In our project this part is used on the A side. Expression is detected with the fluorescence protein mCherry and repressed by Lac repressor.
  
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If needed, you can move this part into B-side plasmid (by DraIII or AatII), regulating the rate of cI/lac repressor.
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This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.
  
 
This part is for our second plan, in case BBa_I751011 doesn't work.
 
This part is for our second plan, in case BBa_I751011 doesn't work.

Latest revision as of 03:29, 27 October 2007

plac-lux-cI-mCherry

In our project this part is used on the A side. Expression is detected with the fluorescence protein mCherry and repressed by Lac repressor.

This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.

This part is for our second plan, in case BBa_I751011 doesn't work.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5246
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2762
    Illegal SpeI site found at 5247
    Illegal PstI site found at 5261
    Illegal NotI site found at 2768
    Illegal NotI site found at 5254
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2762
    Illegal BglII site found at 3016
    Illegal BglII site found at 3698
    Illegal BamHI site found at 2798
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2762
    Illegal XbaI site found at 2777
    Illegal SpeI site found at 5247
    Illegal PstI site found at 5261
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2003
    Illegal SapI site found at 920