Difference between revisions of "Part:BBa K2448014"

Latest revision as of 21:29, 28 October 2017

pPsiA from Sinorhizobium meliloti

This part is the promoter region (0.4 kb upstream) of the PsiA gene of Sinorhizobium meliloti (SMb20484)

pPsiA is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].

Usage and Biology

Our team used this pPsiA promoter together with the Sinorhizobium meliloti PsiR transcription factor (BBa_K2448008) to build a Psicose biosensor (BBa_K2448030). The results show that pPsiA is functional in E. coli and that it behaved as predicted under and without induction.

Using the BPROM webserver [2], the predicted features of this promoter are:
Threshold for promoters -  0.20
Number of predicted promoters -      1
Promoter Pos:    229 LDF-  0.81
-10 box at pos.    214 ctgaatgat Score    31
-35 box at pos.    197 ttgacg    Score    60
Oligonucleotides from known TF binding sites:
No such sites for promoter at    229

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 344
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2448014 short</partinfo>
-.4 kb upstream of PsiA gene of Sinorhizobium meliloti (SMb20484)
+This part is the promoter region (0.4 kb upstream) of the PsiA gene of ''Sinorhizobium meliloti'' (SMb20484)
+pPsiA is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) which is inhibited in the presence of D-Psicose [1].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Our team used this pPsiA promoter together with the ''Sinorhizobium meliloti'' PsiR transcription factor ([[Part:BBa_K2448008|BBa_K2448008]]) to build a Psicose biosensor ([[Part:BBa_K2448030|BBa_K2448030]]). The results show that pPsiA is functional in ''E. coli'' and that it behaved as predicted under and without induction.
+ Using the BPROM webserver [2], the predicted features of this promoter are:
+ Threshold for promoters -  0.20
+ Number of predicted promoters -      1
+ Promoter Pos:    229 LDF-  0.81
+ -10 box at pos.    214 ctgaatgat Score    31
+ -35 box at pos.    197 ttgacg    Score    60
+ Oligonucleotides from known TF binding sites:
+ No such sites for promoter at    229
 <!-- -->