Difference between revisions of "Part:BBa I751011"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I751011 short</partinfo>
 
<partinfo>BBa_I751011 short</partinfo>
This is made for A-side plasmid in our project.
 
  
Regulated by cI repressor, this part makes LuxI, Lac repressor, and mCherry (for detecting).
+
This is made for A-side plasmid in our project.
  
If needed, you can move this part into B-side plasmid (by DraIII or AatII), regulating the rate of cI/lac repressor.
+
Expression is detected with the fluorescence protein mCherry and repressed by lambda cI.
  
 +
This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.
  
  

Latest revision as of 03:26, 27 October 2007

pcI-lux-lac-mCherry on pBR Standard

This is made for A-side plasmid in our project.

Expression is detected with the fluorescence protein mCherry and repressed by lambda cI.

This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2762
    Illegal SpeI site found at 5121
    Illegal PstI site found at 5135
    Illegal NotI site found at 2768
    Illegal NotI site found at 5128
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2762
    Illegal BglII site found at 2890
    Illegal BglII site found at 3572
    Illegal BamHI site found at 2798
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 2762
    Illegal suffix found in sequence at 5121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 2762
    Illegal XbaI site found at 2777
    Illegal SpeI site found at 5121
    Illegal PstI site found at 5135
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2003
    Illegal SapI site found at 920