Difference between revisions of "Part:BBa I751011"
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_I751011 short</partinfo> | <partinfo>BBa_I751011 short</partinfo> | ||
− | |||
− | + | This is made for A-side plasmid in our project. | |
− | + | Expression is detected with the fluorescence protein mCherry and repressed by lambda cI. | |
+ | This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides. | ||
Latest revision as of 03:26, 27 October 2007
pcI-lux-lac-mCherry on pBR Standard
This is made for A-side plasmid in our project.
Expression is detected with the fluorescence protein mCherry and repressed by lambda cI.
This part is designed to move to the B side in our project by digesting with DraIII or AatII to adjaust the repression rate on the both sides.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5120 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2762
Illegal SpeI site found at 5121
Illegal PstI site found at 5135
Illegal NotI site found at 2768
Illegal NotI site found at 5128 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2762
Illegal BglII site found at 2890
Illegal BglII site found at 3572
Illegal BamHI site found at 2798 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2762
Illegal suffix found in sequence at 5121 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2762
Illegal XbaI site found at 2777
Illegal SpeI site found at 5121
Illegal PstI site found at 5135 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2003
Illegal SapI site found at 920