Difference between revisions of "Part:BBa K611016"
 
(6 intermediate revisions by the same user not shown)
Line 5: Line 5:
 
<html>
 
<html>
  
−
This part is can be used as a control for BBa_K611010 and BBa_K611013, which test either C0012 and R0010 mutants respectively.
+
This part is can be used as a control for <a href="https://parts.igem.org/Part:BBa_K611010">BBa_K611010</a> and <a href="https://parts.igem.org/Part:BBa_K611013">BBa_K611013</a>, which test either C0012 and R0010 mutants respectively.
−
</html>
+
  
−
<html>
+
<hr style="background-color: #fff">
−
For 2017, Team UAlberta submitted a new part, <a href="https://parts.igem.org/Part:BBa_K2389070)BBa_K2389070</a>. In this construct, the promoter for LacI was replaced with a constitutive promoter, <a href="https://parts.igem.org/Part:BBa_J23106">BBa_J23106</a>, which eliminates the need to add arabinose as an extra additive.
+
 
 +
<p>For 2017, Team UAlberta submitted <a href="https://parts.igem.org/Part:BBa_K2389070">BBa_K2389070</a>, which replaces the arabinose-regulated promoter for LacI was with a constitutive promoter.</p>
 
</html>
 
</html>
  

Latest revision as of 22:14, 27 October 2017


LacI Repressor and Promoter Wild Type Control

This part is can be used as a control for BBa_K611010 and BBa_K611013, which test either C0012 and R0010 mutants respectively.

For 2017, Team UAlberta submitted BBa_K2389070, which replaces the arabinose-regulated promoter for LacI was with a constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1216
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2394
    Illegal BamHI site found at 1156
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870