Difference between revisions of "Part:BBa K2267029"
(→‎IV Results)
 
(15 intermediate revisions by 4 users not shown)
Line 10: Line 10:
 
This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
 
This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
 
A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri. In vibrio fischeri, the lux box, which is a 20-base inverted repeat unit, is positioned 42.5 bases upstream of the transcriptional start of the lux operon and is required for transcriptional activation.
 
A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri. In vibrio fischeri, the lux box, which is a 20-base inverted repeat unit, is positioned 42.5 bases upstream of the transcriptional start of the lux operon and is required for transcriptional activation.
−
 
−
===design===
 
−
<!-- -->
 
−
Quorum-sensing bacteria such as Vibrio fischeri, are able to detected their own population density and implement density-based decision-making，Using the luxI/luxR quorum-sensing system, synthetic biologists have designed a large number of devices in prokaryotic microorganisms.Inevitably, high performance is required from such devices, and this includes reliability, sensitivity, Hence, to improve the properties of population-density switches, we design of ultrasensitive responses use Point mutation
 
  
 
===Background information===
 
===Background information===
−
Quorum-sensing bacteria such as Vibrio fischeri, are able to detected their own population density and implement density-based decision-making，Using the luxI/luxR quorum-sensing system, synthetic biologists have designed a large number of devices in prokaryotic microorganisms.Inevitably, high performance is required from such devices, and this includes reliability, sensitivity, Hence, to improve the properties of population-density switches, we design of ultrasensitive responses use Point mutation
+
Quorum-sensing bacteria such as Vibrio fischeri, are able to detect their own population density and regulate their growth accordingly.Using the luxI/luxR quorum-sensing system, synthetic biologists have designed a large number of devices in prokaryotic microorganisms.Naturally, high performance is required from such devices, and this includes reliability and sensitivity. Hence, with the goal to improve the properties of population-density switches, we design an ultrasensitive responses base. https://parts.igem.org/Part:BBa_R0062 use Point mutation
 +
 
 
===Experiment Design===
 
===Experiment Design===
  
 +
https://static.igem.org/mediawiki/2017/2/2a/T--TUST_China--part_Experiment_Design.png
  
  
Line 28: Line 26:
  
 
===III Methods===
 
===III Methods===
−
The experiments for the characterization of parts were performed as described previously For density-response testing, cells (E. coli strain BW25113) from single colonies on LB agar (BD, USA) plates were grown overnight in 1 ml nutrition-rich, acid-base equilibrium medium (REM) (15.2 g/l yeast extract (BD, USA), 0.5% (NH4)2SO4, 4 mM MgSO4, 2% glucose and 24 g/l K2HPO4.3H2O, and 9.6 g/l KH2PO4) in Falcon tubes overnight (8−12 h, 1000 rpm, 37 °C, mB100-40 Thermo Shaker, AOSHENG, China). The cultures were subsequently diluted 500-fold with REM in 96-well plates, which were further incubated at 37°C in a shaker at 1000 rpm. Once the diluted cultures reached an OD600 of 0.12–0.14 (~3 h), 10 μL aliquots were transferred into 1 mL REM in 24-well plates (Corning/Costar 3524). These plates were incubated at 37°C in a Varioskan Flash (Thermo Scientific, USA) under constant shaking at 1,000 rpm for 20 h to maintain exponential growth, during which the OD600 and fluorescence values were recorded
+
The experiments for the characterization of parts were performed as described previously for density-response testing. Cells (E. coli strain BW25113) from single colonies on LB agar (BD, USA) plates were grown overnight in 1 ml nutrition-rich, acid-base equilibrium medium (REM) (15.2 g/l yeast extract (BD, USA), 0.5% (NH4)2SO4, 4 mM MgSO4, 2% glucose and 24 g/l K2HPO4.3H2O, and 9.6 g/l KH2PO4) in Falcon tubes overnight (8−12 h, 1000 rpm, 37 °C, mB100-40 Thermo Shaker, AOSHENG, China). The culture media were subsequently diluted 500-fold with REM in 96-well plates, which were further incubated at 37°C in a shaker at 1000 rpm. Once the diluted culture media reach a point where OD600 of 0.12–0.14 (~3 h). 10 μL aliquots were transferred into 1 mL REM in 24-well plates (Corning/Costar 3524). These plates were incubated at 37°C in a Varioskan Flash (Thermo Scientific, USA) under constant shaking at 1,000 rpm for 20 h to maintain exponential growth, during which the OD600 and fluorescence values were recorded at interval.
 +
 
 
===IV Results===
 
===IV Results===
 +
We characterised the activation range of this device using a GFP reporter https://parts.igem.org/Part:BBa_K319039. The results of our characterisation experiments can be found here
 +
we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are
 +
 +
plux1: https://parts.igem.org/Part:BBa_K2267029
 +
 +
plux3: https://parts.igem.org/Part:BBa_K2267030
 +
 +
plux4: https://parts.igem.org/Part:BBa_K2267031
 +
 +
plux6: https://parts.igem.org/Part:BBa_K2267032
 +
 +
plux8: https://parts.igem.org/Part:BBa_K2267046
 +
 +
listed below.
 +
We linked gfp reporter genes
 +
luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043
 +
 +
luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041
 +
 +
luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042
 +
 +
luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044
 +
 +
luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045
  
 +
on them respectively to test the function of modified promoters.
  
 +
https://static.igem.org/mediawiki/2017/e/e6/T--TUST_China--part_part_results.png
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2267029 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2267029 SequenceAndFeatures</partinfo>

Latest revision as of 03:55, 2 November 2017


Plux1

Promoter activated by LuxR in concert with HSL

This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site. A quorum-sensing system involving LuxR, the transcriptional activator, and an acyl-homserine lactone signal regulate the lux operon in vibrio fischeri. In vibrio fischeri, the lux box, which is a 20-base inverted repeat unit, is positioned 42.5 bases upstream of the transcriptional start of the lux operon and is required for transcriptional activation.

Background information

Quorum-sensing bacteria such as Vibrio fischeri, are able to detect their own population density and regulate their growth accordingly.Using the luxI/luxR quorum-sensing system, synthetic biologists have designed a large number of devices in prokaryotic microorganisms.Naturally, high performance is required from such devices, and this includes reliability and sensitivity. Hence, with the goal to improve the properties of population-density switches, we design an ultrasensitive responses base. https://parts.igem.org/Part:BBa_R0062 use Point mutation

Experiment Design

T--TUST_China--part_Experiment_Design.png


part4: https://parts.igem.org/Part:BBa_K2267031

part3: https://parts.igem.org/Part:BBa_K2267030

part1: https://parts.igem.org/Part:BBa_K2267029

III Methods

The experiments for the characterization of parts were performed as described previously for density-response testing. Cells (E. coli strain BW25113) from single colonies on LB agar (BD, USA) plates were grown overnight in 1 ml nutrition-rich, acid-base equilibrium medium (REM) (15.2 g/l yeast extract (BD, USA), 0.5% (NH4)2SO4, 4 mM MgSO4, 2% glucose and 24 g/l K2HPO4.3H2O, and 9.6 g/l KH2PO4) in Falcon tubes overnight (8−12 h, 1000 rpm, 37 °C, mB100-40 Thermo Shaker, AOSHENG, China). The culture media were subsequently diluted 500-fold with REM in 96-well plates, which were further incubated at 37°C in a shaker at 1000 rpm. Once the diluted culture media reach a point where OD600 of 0.12–0.14 (~3 h). 10 μL aliquots were transferred into 1 mL REM in 24-well plates (Corning/Costar 3524). These plates were incubated at 37°C in a Varioskan Flash (Thermo Scientific, USA) under constant shaking at 1,000 rpm for 20 h to maintain exponential growth, during which the OD600 and fluorescence values were recorded at interval.

IV Results

We characterised the activation range of this device using a GFP reporter https://parts.igem.org/Part:BBa_K319039. The results of our characterisation experiments can be found here we did point mutation on https://parts.igem.org/Part:BBa_R0062 and the exact spots are

plux1: https://parts.igem.org/Part:BBa_K2267029

plux3: https://parts.igem.org/Part:BBa_K2267030

plux4: https://parts.igem.org/Part:BBa_K2267031

plux6: https://parts.igem.org/Part:BBa_K2267032

plux8: https://parts.igem.org/Part:BBa_K2267046

listed below. We linked gfp reporter genes luxr-plux1-gfp: https://parts.igem.org/Part:BBa_K2267043

luxr-plux3-gfp: https://parts.igem.org/Part:BBa_K2267041

luxr-plux4-gfp: https://parts.igem.org/Part:BBa_K2267042

luxr-plux6-gfp: https://parts.igem.org/Part:BBa_K2267044

luxr-plux8-gfp: https://parts.igem.org/Part:BBa_K2267045

on them respectively to test the function of modified promoters.

T--TUST_China--part_part_results.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]