Difference between revisions of "Part:BBa I725099:Design"
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===Source=== | ===Source=== | ||
| − | Genes were synthesized from E. coli-optimized Clostridium acetobutylicum sequences from KEGG. | + | Genes were synthesized from ''E. coli''-optimized ''Clostridium acetobutylicum'' sequences from KEGG. |
===References=== | ===References=== | ||
Latest revision as of 21:59, 2 May 2008
Partial operon for butanoate pathway
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 308
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 763
Illegal AgeI site found at 1077
Illegal AgeI site found at 1208
Illegal AgeI site found at 1988
Illegal AgeI site found at 3091
Illegal AgeI site found at 3493
Illegal AgeI site found at 4717 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We were unable to complete addition of the fifth gene to the operon (Butanol dehydrogenase). It needs to be cloned from I725025 to complete this.
Source
Genes were synthesized from E. coli-optimized Clostridium acetobutylicum sequences from KEGG.