Difference between revisions of "Part:BBa K2305011"
 
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[[Image:BIT Figure DNA sequencing K2305011a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011b.png|200px|left|K230500]]
 
[[Image:BIT Figure DNA sequencing K2305011a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011b.png|200px|left|K230500]]
 
[[Image:BIT Figure DNA sequencing K2305011c.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011d.png|200px|left|K230500]]<br clear="all">
 
[[Image:BIT Figure DNA sequencing K2305011c.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011d.png|200px|left|K230500]]<br clear="all">
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==Characterization map==
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[[Image:BIT Figure map K2305011.png|400px|left|K230500]]<br clear="all">
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Fig.The Growth curve of E.coil.
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[[Image:BIT Figure map K2305011a.png|400px|left|K230500]]<br clear="all">
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Fig.The GFP curve of E.coil.
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[[Image:BIT Figure map K2305011b.png|400px|left|K230500]]<br clear="all">
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Fig.The RFP curve of E.coil.
  
  

Latest revision as of 00:26, 28 October 2017


GFP/RFP dual fluorescent system with pbad

GFP/RFP dual fluorescent system with pbad

Agarose gel electrophoresis

We calculated the new part’s base number to be 4352bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

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DNA sequencing

The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

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Characterization map

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Fig.The Growth curve of E.coil.

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Fig.The GFP curve of E.coil.

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Fig.The RFP curve of E.coil.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 4064
    Illegal AgeI site found at 4176
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3062
    Illegal SapI site found at 961