Difference between revisions of "Part:BBa K2305011"

Latest revision as of 00:26, 28 October 2017

GFP/RFP dual fluorescent system with pbad

Agarose gel electrophoresis

We calculated the new part’s base number to be 4352bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

DNA sequencing

The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

Characterization map

Fig.The Growth curve of E.coil.

Fig.The GFP curve of E.coil.

Fig.The RFP curve of E.coil.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2383
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 4064
Illegal AgeI site found at 4176
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 3062
Illegal SapI site found at 961

@@ Line 10: / Line 10: @@
 ==DNA sequencing==
 The figure 2 is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
-[[Image:BIT Figure DNA sequencing K2305011a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011b.png|200px|left|K230500]]<br clear="all">
+[[Image:BIT Figure DNA sequencing K2305011a.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011b.png|200px|left|K230500]]
+[[Image:BIT Figure DNA sequencing K2305011c.png|200px|left|K230500]][[Image:BIT Figure DNA sequencing K2305011d.png|200px|left|K230500]]<br clear="all">
+==Characterization map==
+[[Image:BIT Figure map K2305011.png|400px|left|K230500]]<br clear="all">
+Fig.The Growth curve of E.coil.
+[[Image:BIT Figure map K2305011a.png|400px|left|K230500]]<br clear="all">
+Fig.The GFP curve of E.coil.
+[[Image:BIT Figure map K2305011b.png|400px|left|K230500]]<br clear="all">
+Fig.The RFP curve of E.coil.