Difference between revisions of "Part:BBa K2305003"
 
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==Agarose gel electrophoresis==
 
==Agarose gel electrophoresis==
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We calculated the new part’s base number to be 1007bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
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We calculated the new part’s base number to be 2195bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 
[[Image:BIT Figure DNA sequencing K2305003.png|200px|left|K230500]]<br clear="all">
 
[[Image:BIT Figure DNA sequencing K2305003.png|200px|left|K230500]]<br clear="all">
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==DNA sequencing==
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The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further
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[[Image:BIT Figure DNA sequencing K2305003a.png|200px|left|K230500]]
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[[Image:BIT Figure DNA sequencing K2305003b.png|200px|left|K230500]]<br clear="all">
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==Characterization map==
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[[Image:BIT Figure map K2305003.png|400px|left|K230500]]<br clear="all">
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Fig.The Growth curve of E.coil.
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[[Image:BIT Figure map K2305003a.png|400px|left|K230500]]<br clear="all">
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Fig.The GFP curve of E.coil.
  
  

Latest revision as of 02:06, 28 October 2017


pbad promoter with GFP and lacI

This is a pbad promoter expressing LacI and GFP

Agarose gel electrophoresis

We calculated the new part’s base number to be 2195bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

K230500

DNA sequencing

The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further

K230500
K230500

Characterization map

K230500

Fig.The Growth curve of E.coil.

K230500

Fig.The GFP curve of E.coil.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1303
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1982