Difference between revisions of "Part:BBa K2205005"
 
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<partinfo>BBa_K2205005 short</partinfo>
 
<partinfo>BBa_K2205005 short</partinfo>
  
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The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional.  The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to <i>rhlI</i> (J64718).  This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.<br/><br/>
 
  
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https://static.igem.org/mediawiki/2017/2/2d/--T--Newcastle--MP--Expression_of_GFP_Fim.png
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https://static.igem.org/mediawiki/2017/archive/a/a4/20171027205831%21T--Newcastle--MP_FimON-OFF_diagram.jpeg
  
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Expression of sfGFP over a standard growth curve.  The Fim switch strain was co-cultured with the reporter strain (K2205014) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL.  FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL.  Each data point it the mean of 3 biological replicates.<br/><br/>
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As an improvement on the Tokyo Tech 2015 team part [https://parts.igem.org/Part:BBa_K1632011 BBa_K1632011]The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional.  The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to <i>rhlI</i> (J64718).  This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.<br/><br/>
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https://static.igem.org/mediawiki/2017/0/06/T--Newcastle--iGEM_FimS_Graph_2.png
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Expression of sfGFP over a standard growth curve.  The Fim switch strain was co-cultured with the reporter strain (K2205015) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL.  FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL.  Each data point it the mean of 3 biological replicates.<br/><br/>
 
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<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 21:43, 1 November 2017


Fim Standby Switch


20171027205831%21T--Newcastle--MP_FimON-OFF_diagram.jpeg


As an improvement on the Tokyo Tech 2015 team part BBa_K1632011The Fim switch is a processing module for the Sensynova framework, it is designed to continuously express the red chromoprotein eforRed (K592012) to indicate that the part is functional. The FimE protein (K137007) flips a 300bp promoter region which switches expression from eforRed to rhlI (J64718). This produces the quorum sensing molecule C4-AHL which can be used to activate downstream reporter cells.

T--Newcastle--iGEM_FimS_Graph_2.png


Expression of sfGFP over a standard growth curve. The Fim switch strain was co-cultured with the reporter strain (K2205015) which expresses sfGFP in response to C4-AHL. FimW indicates a white Fim switch reporter in the [ON] state, where the promoter region produces C4-AHL. FimR indicates a red Fim switch reporter in the native [OFF] state which does not produce C4-AHL. Each data point it the mean of 3 biological replicates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]