Difference between revisions of "Part:BBa K2258000"

Latest revision as of 23:15, 1 November 2017

pL and Pleft Hybrid promoter

Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.

Usage and Biology

Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.

Reference

PL Expression System : A Prokaryotic Expression System, Invitrogen manual
Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2258000 short</partinfo>
-pL and Pleft Hybrid promoter
 Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.
+<!-- References -->
+===Reference===
+#PL Expression System : A Prokaryotic Expression System, Invitrogen manual
+#Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU
 <!-- -->