Difference between revisions of "Part:BBa K2271062"

 
 
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__NOTOC__
 
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<partinfo>BBa_K2271062 short</partinfo>
 
<partinfo>BBa_K2271062 short</partinfo>
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===Usage and Biology===
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This part is a composite part comtaining the peroxisomal membrane anchor [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2271104 Pex13] with an N-terminal fusion of the CFP variant mTurquoise. It is applicable for <i>S. cerevisiae</i> using the  HHF1 promoter and the TDH1 terminator. The optimal excitation is at a wavelength of 434 nm with an excitation optimum at 474 nm.
  
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The part contains a peroxisomal membrane protein pex13 with a mTuqoise fused to its n-Terminus.  
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===Experimental Design an Results===
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It can be used as a marker for the peroxisomal membrane in S. cerevisiea with a HHF1 Promotor and TDH1 Terminator.
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The part was used to transform <i>S. cerevisiae</i> BY4742. Transformants were used to validate the part by microscopy using the Elyra PS microscope. The microscope images indicating an localisation of mTurquoise-Pex13 in the peroxisomal membrane (Figure 1).
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[[File:Igemduscgn2017pex13Turqoise.png|500px|thumb|center|'''Figure 1'''The micrscopy showing an localisation of mTurquoise with the typical peroxisomal shape. This indicates, an peroxisomal membrane licalisation of mTurquoise-Pex13]]
  
 
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<partinfo>BBa_K2271062 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2271062 SequenceAndFeatures</partinfo>
  
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===References===
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[1] <b>A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly </b> (2015)  <br>
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Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber <br>
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ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v
  
 
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Latest revision as of 22:30, 31 October 2017


pex13-mTurqoise

Usage and Biology

This part is a composite part comtaining the peroxisomal membrane anchor Pex13 with an N-terminal fusion of the CFP variant mTurquoise. It is applicable for S. cerevisiae using the HHF1 promoter and the TDH1 terminator. The optimal excitation is at a wavelength of 434 nm with an excitation optimum at 474 nm.

Experimental Design an Results

The part was used to transform S. cerevisiae BY4742. Transformants were used to validate the part by microscopy using the Elyra PS microscope. The microscope images indicating an localisation of mTurquoise-Pex13 in the peroxisomal membrane (Figure 1).

Figure 1The micrscopy showing an localisation of mTurquoise with the typical peroxisomal shape. This indicates, an peroxisomal membrane licalisation of mTurquoise-Pex13

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 1987
    Illegal BglII site found at 880
    Illegal BglII site found at 917
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly (2015)
Michael E. Lee, William C. DeLoache, Bernardo Cervantes, and John E. Dueber
ACS Synth. Biol., 2015, 4 (9), pp 975–986 DOI: 10.1021/sb500366v