Difference between revisions of "Part:BBa K2305000"
 
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<partinfo>BBa_K2305000 short</partinfo>
 
<partinfo>BBa_K2305000 short</partinfo>
  
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Strong promoter pcat and promoter plux start the line, luxl generator produce AHL, and AHL combined with luxR products will promote the expression of plux. The lacl products will inhibiting the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, while the GFP express normally.
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Strong constitutive promoter pcat and promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.
 
== Agarose gel electrophoresis ==
 
== Agarose gel electrophoresis ==
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We calculated the new part’s base number to be 4739bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
 
We calculated the new part’s base number to be 4739bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.
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https://static.igem.org/mediawiki/parts/5/50/BIT_Figure_Electrophoretogram_K2305000.png
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[[Image: BIT Figure Electrophoretogram K2305000.png |thumb|center|200px|Fig.1]]<br clear="all">
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==DNA sequencing==
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The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further.
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[[Image:BIT Figure DNA sequencing K2305000.PNG|500px|left|K230500]]<br clear="all">
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==Characterization map==
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[[Image:BIT Figure map K2305000.png|400px|left|K230500]]<br clear="all">
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Fig.The Growth curve of E.coil.
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[[Image:BIT Figure map K2305000a.png|400px|left|K230500]]<br clear="all">
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Fig.The GFP curve of E.coil
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[[Image:BIT Figure map K2305000b.png|400px|left|K230500]]<br clear="all">
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Fig.The RFP curve of E.coil
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===Usage and Biology===
 
===Usage and Biology===
  

Latest revision as of 11:41, 29 October 2017


strong promoter, cycle amplification system and GFP/RFP dual fluorescent system

Strong constitutive promoter pcat and promoter plux stars the circuit, Luxl catalyzes the production of AHL, which will combine with LuxR. Their compound will promote the expression of plux. The Lacl will inhibit the expression of plac promoter to achieve the purpose of inhibiting the expression of RFP, with the amplified expression of GFP on the contrary.

Agarose gel electrophoresis

We calculated the new part’s base number to be 4739bp. The figure1 is the result of agarose gel electrophoresis. We can make a preliminary judgment that the sequences are correct.

Fig.1

DNA sequencing

The figure is a comparison with the results of the sequencing. It verified the accuracy of the sequence further.

K230500

Characterization map

K230500

Fig.The Growth curve of E.coil.

K230500

Fig.The GFP curve of E.coil

K230500

Fig.The RFP curve of E.coil


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1591
    Illegal BglII site found at 2770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4451
    Illegal AgeI site found at 4563
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 64
    Illegal BsaI.rc site found at 3449