Difference between revisions of "Part:BBa K2440036"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR7514 in an Ago2 dependent manner, that is, knockdown of miR7514 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR7514 in an Ago2 dependent manner, that is, knockdown of miR7514 was achieved by transfecting cells with miRNA locker.
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Ghr-miR7514 was firstly detected in in cotton ovules<sup>1</sup>.
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It is also found to be differentially expressed in PHYA1 RNAi cotton and it may be related with the fiber quality of cotton<sup>2</sup>.
  
 
===Sequence and Features===
 
===Sequence and Features===
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This part is sequenced as correct after construction.
 
This part is sequenced as correct after construction.
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==Reference==
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1.A comparative miRNAome analysis reveals seven fiber initiation-related and 36 novel miRNAs in developing cotton ovules.Wang ZM, Xue W, Dong CJ, Jin LG, Bian SM, Wang C, Wu XY, Liu JY Mol Plant. 5:889-900(2012).
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2.Genome-wide identification and characterization of microRNAs differentially expressed in fibers in a cotton phytochrome A1 RNAi line. Miao Q, Deng P, Saha S, Jenkins JN, Hsu CY, Abdurakhmonov IY, Buriev ZT, Pepper A, Ma DP.

Latest revision as of 01:31, 1 November 2017


miR7514 target sequence

It is the target sequence of miR7514, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR7514 in an Ago2 dependent manner, that is, knockdown of miR7514 was achieved by transfecting cells with miRNA locker.

Ghr-miR7514 was firstly detected in in cotton ovules1. It is also found to be differentially expressed in PHYA1 RNAi cotton and it may be related with the fiber quality of cotton2.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.A comparative miRNAome analysis reveals seven fiber initiation-related and 36 novel miRNAs in developing cotton ovules.Wang ZM, Xue W, Dong CJ, Jin LG, Bian SM, Wang C, Wu XY, Liu JY Mol Plant. 5:889-900(2012).

2.Genome-wide identification and characterization of microRNAs differentially expressed in fibers in a cotton phytochrome A1 RNAi line. Miao Q, Deng P, Saha S, Jenkins JN, Hsu CY, Abdurakhmonov IY, Buriev ZT, Pepper A, Ma DP.