Difference between revisions of "Part:BBa K2398022"

 
 
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This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. Together with https://parts.igem.org/Part:BBa_K2398021 this part is needed for the generation of geneIII-deficient M13 bacteriophages. For further information on how to use these parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC. Important information on how to finally produce bacteriophages can also be found the method section (http://2017.igem.org/Team:Heidelberg/Experiments). In contrast to the first part, this part contains a kanamycine resistance for potential selection of the phages after production in follow-up of the transformation into a phage propagation strain containing the plasmid pJC175e.
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Latest revision as of 03:31, 2 November 2017


M13 bacteriophage backbone part 2 w/o gene III for application in Phage-assisted continous evolution


This part is directly related to our Phage-assisted continous evolution (PACE) and Phage-related discontinous evolution (PREDCEL) Toolbox. Together with https://parts.igem.org/Part:BBa_K2398021 this part is needed for the generation of geneIII-deficient M13 bacteriophages. For further information on how to use these parts, please visit our Toolbox guide (http://2017.igem.org/Team:Heidelberg/Toolbox) and our RFC (http://2017.igem.org/Team:Heidelberg/RFC. Important information on how to finally produce bacteriophages can also be found the method section (http://2017.igem.org/Team:Heidelberg/Experiments). In contrast to the first part, this part contains a kanamycine resistance for potential selection of the phages after production in follow-up of the transformation into a phage propagation strain containing the plasmid pJC175e.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2976
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal BsaI.rc site found at 3784