Difference between revisions of "Part:BBa K2448033:Design"

 
 
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<partinfo>BBa_K2448033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2448033 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum
 
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pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]) + RBS ([[Part:BBa_K2448019|BBa_K2448019]]) + D-psicose 3-epimerase from ''Clostridium cellulolyticum'' coding sequence ([[Part:BBa_K2448021|BBa_K2448021]])
  
 
===Source===
 
===Source===
  
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D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum
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DNA synthesis
  
 
===References===
 
===References===
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[1] Mu W, Chu F, Xing Q, Yu S, Zhou L, Jiang B. Cloning, expression, and characterization of a D-psicose 3-epimerase from ''Clostridium cellulolyticum'' H10. J Agric Food Chem. (2011) 59, 7785-7792.
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[2] Chan HC, Zhu Y, Hu Y, Ko TP, Huang CH, Ren F, Chen CC, Ma Y, Guo RT, Sun Y. Crystal structures of D-psicose 3-epimerase from ''Clostridium cellulolyticum'' H10 and its complex with ketohexose sugars. Protein Cell. (2012) 3, 123-131.

Latest revision as of 18:50, 1 November 2017


D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum under the control of pTacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 714
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

pTacI promoter (BBa_K864400) + RBS (BBa_K2448019) + D-psicose 3-epimerase from Clostridium cellulolyticum coding sequence (BBa_K2448021)

Source

DNA synthesis

References

[1] Mu W, Chu F, Xing Q, Yu S, Zhou L, Jiang B. Cloning, expression, and characterization of a D-psicose 3-epimerase from Clostridium cellulolyticum H10. J Agric Food Chem. (2011) 59, 7785-7792.

[2] Chan HC, Zhu Y, Hu Y, Ko TP, Huang CH, Ren F, Chen CC, Ma Y, Guo RT, Sun Y. Crystal structures of D-psicose 3-epimerase from Clostridium cellulolyticum H10 and its complex with ketohexose sugars. Protein Cell. (2012) 3, 123-131.