Difference between revisions of "Part:BBa K2448012"
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<partinfo>BBa_K2448012 short</partinfo> | <partinfo>BBa_K2448012 short</partinfo> | ||
− | pPsiA | + | This part is the promoter region (0.4 kb upstream) of the PsiA gene of ''Sinorhizobium fredii'' (NGR_b11530) |
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+ | pPsiA is predicted to be repressed by the PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) which is inhibited in the presence of D-Psicose [1]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | Our team used this pPsiA promoter together with the ''Sinorhizobium fredii'' PsiR transcription factor ([[Part:BBa_K2448007|BBa_K2448007]]) to build a Psicose biosensor ([[Part:BBa_K2448028|BBa_K2448028]]). The results show that pPsiA is functional in ''E. coli'' and that it behaved as predicted under and without induction. | ||
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+ | Using the BPROM webserver [2], the predicted features of this promoter are: | ||
+ | Threshold for promoters - 0.20 | ||
+ | Number of predicted promoters - 1 | ||
+ | Promoter Pos: 235 LDF- 1.88 | ||
+ | -10 box at pos. 219 cgatagtct Score 37 | ||
+ | -35 box at pos. 197 ttgacg Score 60 | ||
+ | Oligonucleotides from known TF binding sites: | ||
+ | argR2: GCAAATAA at position 244 Score - 6 | ||
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Latest revision as of 21:41, 28 October 2017
pPsiA from Sinorhizobium fredii
This part is the promoter region (0.4 kb upstream) of the PsiA gene of Sinorhizobium fredii (NGR_b11530)
pPsiA is predicted to be repressed by the PsiR transcription factor (BBa_K2448007) which is inhibited in the presence of D-Psicose [1].
Usage and Biology
Our team used this pPsiA promoter together with the Sinorhizobium fredii PsiR transcription factor (BBa_K2448007) to build a Psicose biosensor (BBa_K2448028). The results show that pPsiA is functional in E. coli and that it behaved as predicted under and without induction.
Using the BPROM webserver [2], the predicted features of this promoter are: Threshold for promoters - 0.20 Number of predicted promoters - 1 Promoter Pos: 235 LDF- 1.88 -10 box at pos. 219 cgatagtct Score 37 -35 box at pos. 197 ttgacg Score 60 Oligonucleotides from known TF binding sites: argR2: GCAAATAA at position 244 Score - 6
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 345
- 1000COMPATIBLE WITH RFC[1000]