Difference between revisions of "Part:BBa K2309022:Experience"
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https://static.igem.org/mediawiki/parts/8/82/Dot_blot_for_LL-37_XJTLU-CHINA.jpeg | https://static.igem.org/mediawiki/parts/8/82/Dot_blot_for_LL-37_XJTLU-CHINA.jpeg | ||
| − | The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide produced and right is | + | The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG. |
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| + | For LL-37 ability assay, we tested Minimal Inhibitory concentration(MIC) of <i>Staphylococcus aureus</i>, the result is shown as follows: | ||
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| + | https://static.igem.org/mediawiki/parts/5/5c/LL-37_MIC_XJTLU-CHINA.jpeg | ||
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| + | As picture shows that the concentration of LL-37 from 5ug/ml to 10ug/ml, the LL-37 has highly activities <i>Staphylococcus aureus</i>. | ||
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| + | We also tested the anti-biofilm ability of LL-37 on biofilm formation of <i>Staphylococcus aureus</i>, the result as follows: | ||
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| + | https://static.igem.org/mediawiki/parts/6/6c/LL-37_biofilm_XJTLU-CHINA.jpeg | ||
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| + | The result shows that the value of Absorbance at 595nm decreases while the concentration of LL-37 increases. It seems that LL-37 peptide inhibits <i>Staphylococcus aureus</i> to form the biofilm. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 09:48, 1 November 2017
Applications of BBa_K2309022
This year we use dot blot method to evaluate part K2309022 work.
The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.
For LL-37 ability assay, we tested Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result is shown as follows:
As picture shows that the concentration of LL-37 from 5ug/ml to 10ug/ml, the LL-37 has highly activities Staphylococcus aureus.
We also tested the anti-biofilm ability of LL-37 on biofilm formation of Staphylococcus aureus, the result as follows:
The result shows that the value of Absorbance at 595nm decreases while the concentration of LL-37 increases. It seems that LL-37 peptide inhibits Staphylococcus aureus to form the biofilm.
User Reviews
UNIQbb405ec18696dbd0-partinfo-00000000-QINU UNIQbb405ec18696dbd0-partinfo-00000001-QINU