Difference between revisions of "Part:BBa K2449028"

 
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<partinfo>BBa_K2449028 short</partinfo>
 
<partinfo>BBa_K2449028 short</partinfo>
  
A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1].
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A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. The part [https://parts.igem.org/Part:BBa_K2449029 BBa_K2449029] encodes the toxin RelE.  
  
 
RelB neutralize the toxic effect of RelE through interaction between the two proteins.
 
RelB neutralize the toxic effect of RelE through interaction between the two proteins.
 
RelB was placed under control of the pBAD/AraC promoter in composite part, [https://parts.igem.org/Part:BBa_K2449031 BBa_K2449031].
 
  
 
[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.
 
[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.
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<span class='h3bb'><b>Sequence and Features</b><br>RelB cloned with RBS site and a double terminator.</span>
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<span class='h3bb'><b>Sequence and Features</b><br>RelB cloned with RBS site and a double terminator.<br>RelB was placed under control of the pBAD/AraC promoter in composite part, [https://parts.igem.org/Part:BBa_K2449031 BBa_K2449031].</span>
 
<partinfo>BBa_K2449028 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2449028 SequenceAndFeatures</partinfo>
  

Latest revision as of 07:58, 27 October 2017


RelB with RBS and double terminator

A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In E. coli K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. The part BBa_K2449029 encodes the toxin RelE.

RelB neutralize the toxic effect of RelE through interaction between the two proteins.

[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.


Sequence and Features
RelB cloned with RBS site and a double terminator.
RelB was placed under control of the pBAD/AraC promoter in composite part, BBa_K2449031.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]