Difference between revisions of "Part:BBa K2449028"
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<partinfo>BBa_K2449028 short</partinfo> | <partinfo>BBa_K2449028 short</partinfo> | ||
− | A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. | + | A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In <i>E. coli</i> K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. The part [https://parts.igem.org/Part:BBa_K2449029 BBa_K2449029] encodes the toxin RelE. |
RelB neutralize the toxic effect of RelE through interaction between the two proteins. | RelB neutralize the toxic effect of RelE through interaction between the two proteins. | ||
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[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology. | [1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology. | ||
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− | <span class='h3bb'><b>Sequence and Features</b><br>RelB cloned with RBS site and a double terminator.</span> | + | <span class='h3bb'><b>Sequence and Features</b><br>RelB cloned with RBS site and a double terminator.<br>RelB was placed under control of the pBAD/AraC promoter in composite part, [https://parts.igem.org/Part:BBa_K2449031 BBa_K2449031].</span> |
<partinfo>BBa_K2449028 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2449028 SequenceAndFeatures</partinfo> | ||
Latest revision as of 07:58, 27 October 2017
RelB with RBS and double terminator
A toxin-antitoxin system is a system composed of two gene products, of which one specifies a cell toxin and the other an antitoxin, which neutralizes the toxic effect caused by the toxin. In E. coli K-12 the cytotoxin RelE and antitoxin RelB comprise such a system [1]. The part BBa_K2449029 encodes the toxin RelE.
RelB neutralize the toxic effect of RelE through interaction between the two proteins.
[1] Gotfredsen M, Gerdes K. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Molecular microbiology.
Sequence and Features
RelB cloned with RBS site and a double terminator.
RelB was placed under control of the pBAD/AraC promoter in composite part, BBa_K2449031.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 121
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]