Difference between revisions of "Part:BBa K2333008:Design"

 
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===Design Notes===
 
===Design Notes===
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Stuff
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Stop codons have been removed for the purpose of combination with protein degradation tags. The sequence for this part has been codon-optimized for E. coli.
  
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===Source===
  
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mScarlet-I was originally designed by Bindels et al. 2016 "MScarlet: a bright monomeric red fluorescent protein for cellular imaging". This specific sequence was synthesized by IDT.
  
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===Source===
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We use the website www.jcat.de to optimize the codon sequence for E. coli.
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Nature paper
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===References===
 
===References===
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[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074

Latest revision as of 20:28, 29 October 2017


mScarlet-I no stop codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 512
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Stop codons have been removed for the purpose of combination with protein degradation tags. The sequence for this part has been codon-optimized for E. coli.

Source

mScarlet-I was originally designed by Bindels et al. 2016 "MScarlet: a bright monomeric red fluorescent protein for cellular imaging". This specific sequence was synthesized by IDT.

We use the website www.jcat.de to optimize the codon sequence for E. coli.

References

[1] Bindels, D. S., Haarbosch, L., Weeren, L. V., Postma, M., Wiese, K. E., Mastop, M., . . . Gadella, T. W. (2016). MScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nature Methods, 14(1), 53-56. doi:10.1038/nmeth.4074