Difference between revisions of "Part:BBa K2485012"
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<partinfo>BBa_K2485012 short</partinfo> | <partinfo>BBa_K2485012 short</partinfo> | ||
| − | This is a triple fusion protein containing green light absorbing proteorhodopsin, the cPT region of IcsA, and Fresno RFP. It is intended to function as a unipolarly expressing proton pump. The cPT region directs the localization of the protein to the old pole of E. Coli while the proteorhodopsin pumps protons out of the cell in response to green light (must be grown in 10uM all-trans retinal). RFP has also been tagged for fluorescent visualization. We also have referred to this as the Voltage protein. | + | This is a triple fusion protein containing green light absorbing proteorhodopsin, the cPT region of IcsA, and Fresno RFP. It is intended to function as a unipolarly expressing proton pump. The cPT region directs the localization of the protein to the old pole of E. Coli while the proteorhodopsin pumps protons out of the cell in response to green light (must be grown in 10uM all-trans retinal). RFP has also been tagged for fluorescent visualization. We also have referred to this as the Voltage protein. See [https://parts.igem.org/Part:BBa_K2485013 BBa_K2485013] for characterization. Note that this part improves upon part [https://parts.igem.org/Part:BBa_K773002 BBa_K773002] from the 2012 Caltech iGEM by adding multiple functional domains. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2485012 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2485012 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Add more about the biology of this part here --> | ||
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Latest revision as of 06:09, 27 October 2017
Proteorhodopsin-cPT-RFP
This is a triple fusion protein containing green light absorbing proteorhodopsin, the cPT region of IcsA, and Fresno RFP. It is intended to function as a unipolarly expressing proton pump. The cPT region directs the localization of the protein to the old pole of E. Coli while the proteorhodopsin pumps protons out of the cell in response to green light (must be grown in 10uM all-trans retinal). RFP has also been tagged for fluorescent visualization. We also have referred to this as the Voltage protein. See BBa_K2485013 for characterization. Note that this part improves upon part BBa_K773002 from the 2012 Caltech iGEM by adding multiple functional domains.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1117
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]