Difference between revisions of "Part:BBa I746210:Experience"

Latest revision as of 21:45, 24 October 2007

It is not known whether this device actually causes cells to generate and export usable AIP or not, since an assay for AIP was never designed. They were tested with the reciever device built (I746220) but this produced a negative result. This was done in several ways. Firstly, overnight cultures of recievers were diluted 1:100, grown for 2 hours and then induced with varying amounts of supernatant from overnight sender cultures. They were viewed after two hours on a fluorescence microscope. A variation on this was to induce and then use a plate reader to take readings. Finally, a lawn of recievers was prepared by diluting 100ul of culture into 3ml of molten top agar, and induction was by spotting supernatant or sender cell culture onto a filter paper disc in the centre of the lawn. None of these tests showed induction.

It is not known which part of the system is at fault.

Applications of BBa_I746210

User Reviews

UNIQbf67b7182f006a9d-partinfo-00000000-QINU UNIQbf67b7182f006a9d-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
+It is not known whether this device actually causes cells to generate and export usable AIP or not, since an assay for AIP was never designed. They were tested with the reciever device built ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I746220 I746220]) but this produced a negative result. This was done in several ways. Firstly, overnight cultures of recievers were diluted 1:100, grown for 2 hours and then induced with varying amounts of supernatant from overnight sender cultures. They were viewed after two hours on a fluorescence microscope. A variation on this was to induce and then use a plate reader to take readings. Finally, a lawn of recievers was prepared by diluting 100ul of culture into 3ml of molten top agar, and induction was by spotting supernatant or sender cell culture onto a filter paper disc in the centre of the lawn. None of these tests showed induction.
+It is not known which part of the system is at fault.
 ===Applications of BBa_I746210===