Difference between revisions of "Part:BBa K2440018"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-384 in an Ago2 dependent manner, that is, knockdown of miR-384 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-384 in an Ago2 dependent manner, that is, knockdown of miR-384 was achieved by transfecting cells with miRNA locker.
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Hsa-miR-384 was firstly found in human and rat.<sup>1</sup>
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MiR-384 was significantly downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. The overexpression of miR-384 repressed the growth and invasion of NSCLC cells, whereas its suppression showed the opposite effect. Moreover, astrocyte elevated gene-1 (AEG-1) was identified as a target gene of miR-384. The overexpression of miR-384 significantly decreased AEG-1 expression and Wnt signaling, whereas its suppression promoted this pathway. Furthermore, miR-384 was inversely correlated with AEG-1 expression in NSCLC tissues. Additionally, restoration of AEG-1 expression in miR-384-overexpressing cells significantly reversed the antitumor effects of miR-384.<sup>2</sup>
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Moreover, miR-384 suppresses the growth and invasion of Renal Cell Carcinoma Cells (RCC) via downregulation of AEG-1, providing a potential therapeutic target for treatment of RCC.<sup>3</sup>
  
 
===Sequence and Features===
 
===Sequence and Features===
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This part is sequenced as correct after construction.
 
This part is sequenced as correct after construction.
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==Reference==
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1.A pancreatic islet-specific microRNA regulates insulin secretion. Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).
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2.MicroRNA-384 represses the growth and invasion of non-small-cell lung cancer by targeting astrocyte elevated gene-1/Wnt signaling.Fan N, Zhang J, Cheng C, Zhang X, Feng J, Kong R.
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3.MicroRNA-384 Inhibits the Growth and Invasion of Renal Cell Carcinoma Cells by Targeting Astrocyte Elevated Gene 1.Song H, Rao Y, Zhang G, Kong X.

Latest revision as of 00:58, 1 November 2017


miR-384 target sequence

It is the target sequence of miR-384, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-384 in an Ago2 dependent manner, that is, knockdown of miR-384 was achieved by transfecting cells with miRNA locker.

Hsa-miR-384 was firstly found in human and rat.1

MiR-384 was significantly downregulated in non-small-cell lung cancer (NSCLC) tissues and cell lines. The overexpression of miR-384 repressed the growth and invasion of NSCLC cells, whereas its suppression showed the opposite effect. Moreover, astrocyte elevated gene-1 (AEG-1) was identified as a target gene of miR-384. The overexpression of miR-384 significantly decreased AEG-1 expression and Wnt signaling, whereas its suppression promoted this pathway. Furthermore, miR-384 was inversely correlated with AEG-1 expression in NSCLC tissues. Additionally, restoration of AEG-1 expression in miR-384-overexpressing cells significantly reversed the antitumor effects of miR-384.2

Moreover, miR-384 suppresses the growth and invasion of Renal Cell Carcinoma Cells (RCC) via downregulation of AEG-1, providing a potential therapeutic target for treatment of RCC.3

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.A pancreatic islet-specific microRNA regulates insulin secretion. Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).

2.MicroRNA-384 represses the growth and invasion of non-small-cell lung cancer by targeting astrocyte elevated gene-1/Wnt signaling.Fan N, Zhang J, Cheng C, Zhang X, Feng J, Kong R.

3.MicroRNA-384 Inhibits the Growth and Invasion of Renal Cell Carcinoma Cells by Targeting Astrocyte Elevated Gene 1.Song H, Rao Y, Zhang G, Kong X.