Difference between revisions of "Part:BBa K2381013"
 
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<partinfo>BBa_K2381013 short</partinfo>
 
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<p>this part can response to blue light (470nm), rapidly bind to the corresponding parts, <partinfo>BBa_K2381014</partinfo>, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.  
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this part can response to blue light (470nm), rapidly bind to the corresponding parts, <partinfo>BBa_K2381014</partinfo>, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.  
 
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<img src="https://static.igem.org/mediawiki/parts/d/df/HZAU_2017_MagLuc.png" width="700px"/>
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<b>Fig.1 </b>Prove the function of pMag and nMag. n=1
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2381013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2381013 SequenceAndFeatures</partinfo>
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Latest revision as of 03:24, 2 November 2017


nMag-LucC

this part can response to blue light (470nm), rapidly bind to the corresponding parts, BBa_K2381014, due to eletrostatic interaction and dimerize. Its cofactor for light sensing is flavin adenine dinucleotide (FAD). After dimerization the split luciferase will complement to recover its abillity to generate fluorescenece.


Fig.1 Prove the function of pMag and nMag. n=1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 762
    Illegal AgeI site found at 901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403