Difference between revisions of "Part:BBa K2477010"

Latest revision as of 22:52, 26 October 2017

TLP Secretion System

This circuit constitutively expresses the antifungal agent, TLP, tagged with a 6x His-tag, a 2x GS linker, and an HlyA secretion peptide. The HlyA tag allows for secretion using the HlyB/HlyD system. Both HlyB and HlyD are also encoded on this part and are under control of an arabinose promoter. Please note that there is no scar site between the end of TLP (the GSGS linker) and the start of the HlyA tag.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2180
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2119
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 181
Illegal AgeI site found at 284
Illegal AgeI site found at 326
Illegal AgeI site found at 1954
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 468
Illegal SapI site found at 1936

Revision as of 22:46, 26 October 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 22:52, 26 October 2017 (view source) Jpremo (Talk \| contribs) m
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	<partinfo>BBa_K2477010 short</partinfo>		<partinfo>BBa_K2477010 short</partinfo>

−	This circuit constitutively expresses the antifungal agent, TLP, tagged with a 6x His-tag, a 2x GS linker, and an HlyA secretion peptide. The HlyA tag allows for secretion using the HlyB/HlyD system. Both HlyB and HlyD are also encoded on this part and are under control of an arabinose promoter.	+	This circuit constitutively expresses the antifungal agent, TLP, tagged with a 6x His-tag, a 2x GS linker, and an HlyA secretion peptide. The HlyA tag allows for secretion using the HlyB/HlyD system. Both HlyB and HlyD are also encoded on this part and are under control of an arabinose promoter. Please note that there is no scar site between the end of TLP (the GSGS linker) and the start of the HlyA tag.